Xiaoya Liu is an Attending Physician at, the Department of Oncology, the First Affiliated Hospital of Chongqing Medical University, china. She completed Resident Physician, Dept. of Oncology, at The First Affiliated Hospital of Chongqing Medical University. She is a Member of the Chinese Anti-Cancer Association.
MicroRNA (miRNA) is reported to be closely related to a variety of pathophysiological processes of carcinoma and is considered a potential biomarker for the diagnosis of lung cancer with brain metastasis（LCBM）. But, it will be a huge challenge to develop an accurate and sensitive miRNA detection method. We develop here a novel approach for sensitive and accurate detection of miRNA through integrating garland RCA (rolling circle amplification) and CRISPR-Cas9-assisted signal generation. In this method, the target miRNA cyclized the dumbbell padlock and triggered the RCA process to form long single-strand DNA products with repeated hairpin structures. Upon the addition of a complementary sequence, a double strand-DNA sequence (dsDNA) was formed. With the assistance of the Cas9 enzyme for specific recognition and cleavage of formed dsDNA, the RCA products were disassembled into hairpin probes. the generated hairpin probe could be unfolded by the target miRNA and thus initiate the CHA (catalytic hairpin assembly) process for signal generation. By integrating the RCA and CHA process, the method eventually exhibited a favorable detection performance. In general, we have faith in that this method is a creative approach that is suitable for being applied in the screening, diagnosis, and prognostication of LCBM.